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Abstract
The nucleotide sequence of the glyoxalase I gene of Pseudomonas putida IFO 3738 was determined. The glyoxalase I gene coded for a polypeptide of 164-amino acids with a calculated molecular weight of 18,442, which was closely similar to that obtained with purified glyoxalase I (M.W. 19,500) from P. putida. The gene for glyoxalase I was expressed in Escherichia coli cells. Comparison of the nucleotide sequence of the gene with the N-terminal amino acid sequence of the enzyme synthesized in E. coli cells revealed that the N-terminal methionine residue was removed after translation, possibly by methionine aminopeptidase in E. coli. The glyoxalase I contained 1 zinc atom per enzyme and its pI was 4.0.