Abstract
A taxonomic study below the generic or at the specific level was performed as to the electrophoretic patterns of seven enzymes in twenty-six strains of Lipomyces and Waltomyces species. The seven enzymes were glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, hexokinase, phosphoglucomutase and fumarase. The five species, L. anomalus, L.kononenkoae, L. starkeyi, L. tetrasporus and W. lipofer constituted separate clusters from each other, the similarity values being all 0%. The two strains of L. kononenkoae were quite dissimilar in their electrophoretic enzyme patterns (s = 0%). The unidentified Lipomyces strain, CBS 8113, was very similar to the type strain of L. kononenkoae (s = 100%). The five strains of L. starkeyi were divided into two groups with a similarity value of 0%. The unidentified Lipomyces strain, CBS 8064, was a member of the second L. starkeyi group (s = 43%). The eleven strains of L. tetrasporus were divided into three separate groups with similarity values of less than 30%. The four strains of W. lipofer had identical electrophoretic enzyme patterns (s = 100%). The unidentified Lipomyces strain, CBS 8114, constituted a separate cluster, which was linked to the four strains of W. lipofer with a similarity value of only 14%. These data are discussed from the taxonomic point of view.