High-Speed Separation of Oleanolic acid and Ursolic acid from the Fruit of Ligustrum Lucidum ait and Crataegus by Cyclodextrin-Modified Micellar Electrokinetic Chromatography

ABSTRACT

A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique for determining oleanolic acid and ursolic acid in the fruit of Ligustrum lucidum Ait and Crataegus was developed. The two bio-active components were successfully separated within 4 minutes using a pH 8.5 sodium borate buffer containing 60mM SDS and 2mM γ-CD. The correlation coefficients of the linear calibration graphs for the analyses exceeded 0.9980. The effects of pH, SDS, γ-CD, sodium borate and temperature on separation were also investigated.

Keywords::

• Separation
• micellar electrokinetic chromatography
• oleanolic acid
• ursolic acid