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ABSTRACT
A simple and accurate procedure for indirect spectrofluorimetric determination of glycerol and ethylene glycol in aqueous media was developed. Alizarin Navy Blue can be oxidized by potassium periodate to produce a fluorescent compound, that can be detected fluorimetrically (λex = 370 nm, λem = 516 nm) by a flow through method. Glycerol and ethylene glycol react with periodate decreasing its concentration and thus the forming the basis for an indirect determination of these compounds. The influence of acid concentration, reagent concentration and manifold variables were studied. For glycerol and ethylene glycol linear calibration curves were obtained in the ranges of 4.8 × 10-7 – 8.7 × 10-5 M and 6.4 × 10-7 – 8.7 × 10-5 M, respectively. The limit of detection (defined as the concentration that gives a signal three times the standard deviation of the background signal) for glycerol and ethylene glycol was 3.2 × 10-7 M and 4.3 × 10-7 M, respectively. The proposed method was applied for determination of glycerol and ethylene glycol in synthetic and real samples. The results obtained were satisfactory.