Abstract
Simultaneous determination of cysteine and homocysteine in binary mixtures was performed by application of neural networks on the spectral kinetic data. This method is based on the complexation of bivalent iron with 2,2′–bipyridin (bipy). Iron(III) is quantitatively reduced to iron(II) with cysteine and homocysteine in the presence of 2,2′–bipyridin producing iron(II)–bipy complex (λmax=522 nm), and it can be used as a visible spectrophotometric signal for indirect simultaneous determination of the cysteine and homocysteine concentrations. On the basis of the difference in the rate between the two reactions, these two amino acids can be determined simultaneously using principal component‐artificial neural networks (PC‐ANN). The parameters controlling behavior of the system were investigated and optimum conditions selected. Determinations were made over the concentration range 0.10–5.50 µg · mL−1 of cysteine and 0.1–5.00 µg · mL−1 of homocysteine. Applying this method satisfactorily to simultaneous determination of these amino acids with total relative standard error less than 5% validated the proposed method.
We gratefully acknowledge the support of this work by the Shiraz University Research Council.