Comparative Analysis of Medroxyprogesterone Acetate Residue in Animal Tissues by ELISA and GC‐MS

Abstract

Medroxyprogesterone acetate is a xenobiotic growth promoter of meat‐producing animals. In this study, the liver, kidney, and muscle of rabbits were obtained after the medroxyprogesterone acetate was administrated successively for 5 days at 0.8 mg/kg and withdrawn for 7 days before slaughter. The medroxyprogesterone acetate residues were determined by ELISA method. The average residue in muscle, liver, and kidney, as 7, 6, and 23 µg/kg, respectively. Residues were confirmed by GC/MS by monitoring four ions, 373, 283, 265, and 145 m/z. A good correlation was observed between the GC/MS and ELISA methods.

Keywords:

• Medroxyprogeterone acetate
• residue
• ELISA
• GC/MS

Acknowledgments

This work was financially supported by the National Science Foundation of China (No. 20475022). The authors thank Mr. Zhu Jian for helping us with the GC‐MS analysis in the Shanghai Entry‐Exit inspection and Quarantine Bureau.