Adsorptive Stripping Voltammetric Behavior of Adenosine Triphosphate (ATP) in Presence of Copper at the Mercury Film Electrode

Abstract

A stripping method for the determination of adenosine triphosphate (ATP) in presence of copper at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation of ATP‐copper at thin‐film mercury electrode followed by linear DC or cyclic voltammetry scan measurement of the surface species. Optimum experimental conditions were found to be the use of a 1×10⁻³ M NaOH solution, an accumulation potential of −0.40 V and a scan rate of 200 mV · s⁻¹. The response of ATP‐copper is linear over the concentration range 0.2–1.2 ppm. For an accumulation time of 30 min, the detection limit was found to be 14 ppb (2.5×10⁻⁸ M). The more convenient relation to measuring the ATP in presence of metals, adenine and amino acids were also investigated.

Keywords:

• Adenosine triphosphate (ATP) determination
• copper
• adenine
• DNA
• amino acids
• thin‐film mercury electrode
• stripping voltammetry

Acknowledgments

The authors gratefully acknowledge the CNPq and CAPES of the Government of Brazil and PUC‐Rio for support of this work. The experimental assistance of A.B. Neves is appreciated