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Abstract
A new, simple, and sensitive reverse‐phase liquid chromatographic method was developed and validated for the estimation of Lercanidipine hydrochloride in rabbit serum using UV detector under isocratic conditions. After subjecting serum to simple and efficient one‐step extraction procedure, 100 µl of sample was injected onto high‐performance liquid chromatography system. The detector response was linear in the concentration range of 25–1000 ng/ml. The developed method was validated as per standard guidelines. Validation demonstrated accuracy, precision, and selectivity of the proposed method. The drug was found to be stable under various processing and storage conditions.
Acknowledgments
The authors are grateful to the University Grants Commission (UGC), New Delhi for their financial support. The authors are thankful to Glenmark Pharmaceuticals Limited, Mumbai for providing lercanidipine hydrochloride as a gift sample.