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Biosensors

Amperometric Glucose Biosensor Based on Ultrafine Platinum Nanoparticles

, , , &
Pages 2116-2127 | Received 13 May 2007, Accepted 15 Jun 2007, Published online: 09 Nov 2007
 

Abstract

In the present paper the ultrafine and highly dispersed platinum nanoparticles (average size 3 nm) were used for the construction of a glucose biosensor in a simple method. An excellent response to glucose has been obtained with a high sensitivity (137.7 µA mM−1 cm−2) and fast response time (5 s). The biosensor showed a detection limit of 5 µM (at the ratio of signal to noise, S/N=3) and a linear range form 0.2 to 3.2 mM with a correlation coefficient r=0.999. The apparent Michaelis–Menten constant (k m) and the maximum current were estimated to be 9.36 and 1.507 mA mM−1 cm−2, respectively. In addition, effects of pH value, applied potential and the interferents on the amperometric response of the sensor were investigated and discussed.

Acknowledgments

The authors wish to express their gratitude and appreciation for the financial support from the National Natural Science Foundation of China (No. 50671098 and 20473091).

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