Chromatographic Fingerprint Analysis of the Flowers of Abelmoschus manihot Using HPLC with Photodiode Array Detection

Abstract

A simple and valid chromatographic fingerprint analysis method was developed using high‐performance liquid chromatography‐photodiode array detection for the quality analysis of the flowers of Abelmoschus manihot. For the first time, the feasibility and advantages of employing chromatographic fingerprint were investigated and demonstrated for the evaluation of the flowers of A. manihot by systematically comparing chromatograms with professional analytical software recommended by State Food and Drug Administration. Our results revealed that the chromatographic fingerprint combining similarity evaluation could be used efficiently for the identification and quality assessment of raw herbs of the flowers of A. manihot from different sources.

Keywords:

• Chromatographic fingerprint
• quality analysis
• Abelmoschus manihot
• HPLC

Notes

This work is supported financially by the National Natural Science Foundation of China (Grant No. 20432030) and the program for Changjiang Scholar and Innovative Team in University (Grant No. 985‐2‐063‐112).