Amperometric Glucose Sensor Fabricated by Combining Glucose Oxidase Micelle Membrane and Aminated Glassy Carbon Electrode

Abstract

An amperometric glucose biosensor based on the detection of the reduction of oxygen has been developed by combining an aminated glassy carbon electrode with a polystyrene (PS) membrane containing glucose oxidase (GOD) micelles. The structure of GOD micelles contained in PS membrane was observed by scanning electron microscope. The micelle has a roughly spherical shape, and the enzyme colony is contained inside the micelle. This glucose sensor exhibited good sensitivity with short response time (within 2 min). A good linear relationship was observed in the concentration range of 0.2 mM to 2.6 mM when the applied potential was − 0.45 V vs. Ag/AgCl.

Keywords:

• Aminated glassy carbon electrode
• Glucose biosensor
• Glucose oxidase micelle
• Polystyrene membrane
• Polymaleimidostyrene