Electrochemiluminescence Detection of Clarithromycin in Biological Fluids after Capillary Electrophoresis Separation

Abstract

A sensitive analytical method for the determination of clarithromycin in biological fluids with electrochemiluminescence detection after capillary electrophoresis separation was proposed in this paper, based on the enhancing effect of clarithromycin on electrochemiluminescence of tris(2,2-bipyridyl) ruthenium(II) (Ru(bpy)₃ ²⁺). Various factors influencing the separation and detection of clarithromycin were examined to establish the detection system. Under the optimized conditions, the electrochemiluminescence intensity is proportional with the concentration of the analyte over the range of 0.1–10 µM with a detection limit of 30 nM (S/N = 3). The proposed method has been successfully applied for clarithromycin content determination in spiked human plasma and urine samples.

Keywords:

• Biological fluids
• Capillary electrophoresis-electrochemiluminescence (CE-ECL)
• Clarithromycin

This work was partly supported from “863” National High Technology Research Development Program of China (No. 2008AA10Z419), National Natural Science Foundation of China (No. 30571347, 30671525), MOE High School Doctoral Programmes Founding for New Teachers (No. 20070295014), and the Science Foundation of Jiangnan University, China.

Notes

Note. Six replicates per day, on 3 different days.