Sensitive Detection of NADH by Ferrocenylalkanethiol Functionalized Multiwall Carbon Nanotubes Electrodes

Abstract

The 6-ferrocenylhexanethiol (FcC₆SH) functionalized multiwall carbon nanotubes (MWNTs) modified glassy carbon electrode (FcC₆SH/MWNTs/GCE) was easily fabricated and used for the sensitive detection of NADH. Cyclic voltammetric and amperometric methods were used to study the behavior of NADH on the FcC₆SH/MWNTs/GCE. A broader linear response range to the NADH concentration from 5 µM to 1.5 mM with a correlation coefficient of 0.9982 was obtained. The detection limit was 0.54 µM. The synergetic effects of FcC₆SH and MWNTs make the modified electrode highly sensitive to NADH. In addition, the modified electrode can decrease the fouling of the electrode surface.

Keywords:

• Ferrocenylhexanethiol
• Multiwall carbon nanotube
• Modified electrode
• NADH

This work was supported by grants from the Ministry of Science and Technology of China (973 Program No. 2006CB705601) and the National Natural Science Foundation of China (No. 20705038).