78
Views
1
CrossRef citations to date
0
Altmetric
ELECTROCHEMISTRY

Histidine Determination in the Presence of Copper in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

, , , &
Pages 1248-1266 | Received 17 Jan 2008, Accepted 01 Mar 2008, Published online: 16 Jun 2010
 

Abstract

A stripping method for the determination of histidine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation of histidine-copper complex at mercury film electrode followed by linear cyclic scan voltammetry measurement of the surface species. Optimum experimental conditions were found to be the use of a 1.0 × 10−3 M NaOH solution, an accumulation potential of − 0.20 V, and a linear scan rate of 200 mV sec−1. The response of histidine is linear over the concentration range 0.02–0.12 ppm. For an accumulation time of 20 min, the detection limit was found to be 0.5 ppb (3.2 × 10−9 M). The more convenient relation to measuring the histidine in the presence of metals, cysteine, tyrosine, and other amino acids was also investigated. The utility of the method is demonstrated by presence of casein, ATP, and ss DNA.

The authors gratefully acknowledge the CNPq and CAPES of the Government of Brazil and PUC-Rio for support of this work. The experimental assistance of A.B. Neves is appreciated.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.