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Abstract
A sequential injection method coupled to chemiluminescence detection was described for the determination of trace amount of histidine. The physical and chemical parameters depicting the system were studied to obtain optimum conditions. It was found that physical dispersion caused by the change of zone stacking sequence was significant factor influencing CL intensity for a rapid CL reaction. At optimized conditions, histidine can be determined in the linear range from 5.0 × 10−7to 1.0 × 10−3 M with a detection limit (3σ) of 2 × 10−7 M for 60 µl sample. The relative standard deviation (RSD) for eleven repeated measurements of 4 × 10−5 M histidine was 0.97%, and the sampling frequency was 80 h−1, and the recoveries were varied from 90.0 to 103.3%. The proposed method has been successfully applied to the determination of histidine in beer samples.
This work was supported by the National Nature Science Foundation of China Project (No. 20077028).