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BIOANALYTICAL

Simultaneous Determination of Hydrochlorothiazide and Valsartan in Human Plasma by Liquid Chromatography/Tandem Mass Spectrometry

, , , &
Pages 1348-1365 | Received 01 Mar 2008, Accepted 18 Mar 2008, Published online: 10 Jul 2008
 

Abstract

A rapid and specific liquid chromatography/tandem mass spectrometry method was described for the simultaneous determination of hydrochlorothiazide and valsartan in human plasma. After extracted from plasma using methanol, hydrochlorothiazide, valsartan and hydroflumethiazide, irbesartan, used as the internal standard, respectively, were chromatographically analyzed on a Phenomenex Kromasil C8 column with water and methanol (27:73, v/v) as the mobile phase. Selected reaction monitoring was specific for mass detection employing negative electrospray ionization. The calibration standards were linear over the concentration range (3.13–800 ng/ml for hydrochlorothiazide and 11.72–3000 ng/ml for valsartan). The method was found to be suitable for application to a pharmacokinetic study after oral administration of dispersible tablet containing 12.5 mg hydrochlorothiazide and 80 mg valsartan to 20 healthy volunteers.

The authors gratefully acknowledge financial support from the NSFC (No. 20332020) and University of Science and Technology of China.

Notes

RSD: Relative standard deviation; RE: Relative error.

RSD: Relative standard deviation; RE: Relative error; r.t.: Room temperature.

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