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LUMINESCENCE

Selective Determination of Harmol by Room-Temperature Phosphorimetry: A Comparative Performance with Micellar Electrokinetic Capillary Chromatography

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Pages 1648-1657 | Received 10 Oct 2007, Accepted 15 Feb 2008, Published online: 17 Jul 2008
 

Abstract

Selective determination of harmol in the presence of other β-carboline alkaloids without the need for previous separation of components was achieved by Solid Surface Room-Temperature Phosphorimetry (SSRTP) using HgCl2. Detection of harmol at concentrations as low as 5.2 × 10−7 mol L−1 can be made in urine samples. Recovery of 100±12% was achieved. The analytical performance of SSRTP was compared to Micellar Electrokinetic Capillary Chromatography (MECC).

Financial support from the Brazilian agencies FAPERJ and FINEP is gratefully acknowledged. Aucélio, Figueiredo, and de Souza thank CNPq, and Marques thanks CAPES and FAPERJ for scholarships.

Notes

Iharmol in mixture/Iharmol: Ratio between RTP measured from 2 nmol of harmol from the β-carboline mixture and the RTP measured from 2 nmol of harmol from a standard solution.

a Concentration or mass of harmol producing analytical signal is equal to 3 times the blank signal.

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