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SEPARATIONS AND HPLC

Evaluation of Monolithic Columns for Determination of Formaldehyde and Acetaldehyde in Sugar Cane Spirits by High-Performance Liquid Chromatography

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Pages 1674-1681 | Received 10 Oct 2007, Accepted 18 Feb 2008, Published online: 17 Jul 2008
 

Abstract

High-Performance Liquid Chromatography (HPLC) conditions are described for separation of 2,4-dinitrophenylhydrazone (2,4-DNPH) derivatives of carbonyl compounds in a 10 cm long C18 reversed phase monolithic column. Using a linear gradient from 40 to 77% acetonitrile (acetonitrile-water system), the separation was achieved in about 10 min—a time significantly shorter than that obtained with a packed particles column. The method was applied for determination of formaldehyde and acetaldehyde in Brazilian sugar cane spirits. The linear dynamic range was between 30 and 600 µg L−1, and the detection limits were 8 and 4 µg L−1 for formaldehyde and acetaldehyde, respectively.

Financial support and fellowships from FAPESP and CNPq are gratefully acknowledged.

Notes

a,b LOD and LOQ were defined at the signal to noise ratios of 3 : 1 and 10 : 1, respectively.

a The confidence limits were estimated at the 95% confidence level from the statistical parameters of the analytical curve (Miller and Miller Citation1988), considering a triplicate (n = 3) of experiments.

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