A Direct Chemiluminescence Method for the Determination of Prulifloxacin Using Tris-(4,7-Diphenyl-1,10-Phenanthrolinedisulfonic Acid) Ruthenium(II)–Cerium(IV) System

Abstract

A simple, rapid, injection chemiluminescence method is described for the determination of prulifloxacin, a commonly used antibiotic. A strong chemiluminescence signal was detected when a mixture of the analyte and tris-(4,7-diphenyl-1,10-phenanthrolinedisulfonic acid)ruthenium(II) was injected into cerium(IV) sulfate. The chemiluminescence signal is proportional to the concentration of prulifloxacin in the range 4.0 × 10⁻⁸–9.0 × 10⁻⁶ mol L⁻¹. The detection limit is 1.0 × 10⁻⁸ mol L⁻¹, and the relative standard deviation is 2.2% (n = 11) for the determination of 8.0 × 10⁻⁷ mol L⁻¹ prulifloxacin. The proposed method was successfully applied to the determination of prulifloxacin in pharmaceutical preparations in capsules, spiked serum, and urine samples.

Keywords:

• Chemiluminescence
• cerium(IV)
• flow-injection
• prulifloxacin
• tris-(4
• 7-diphenyl-1
• 10-phenanthrolinedisulfonic acid)ruthenium(II)

The authors acknowledge support from the National Natural Science Foundation of China, the Science Foundation of Huazhong University of Science & Technology (0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.

Notes

^a From Zhengda Fine Pharmaceutical Co. Ltd., Jiangsu, China (No. H20060830).

^b Mean±sd, Average of five determinations.

Note. RSD, relative standard deviation.

^a From a healthy volunteer man in local hospital, Huaibei, China.

^b Mean±SD, Average of five determinations.