Direct Electroanalytical Determination of Fluvastatin in a Pharmaceutical Dosage Form: Batch and Flow Analysis

Abstract

The reduction of luvastatin (FLV) at a hanging mercury-drop electrode (HMDE) was studied by square-wave adsorptive-stripping voltammetry (SWAdSV). FLV can be accumulated and reduced at the electrode, with a maximum peak current intensity at a potential of approximately −1.26 V vs. AgCl/Ag, in an aqueous electrolyte solution of pH 5.25. The method shows linearity between peak current intensity and FLV concentration between 1.0 × 10⁻⁸ and 2.7 × 10⁻⁶ mol L⁻¹. Limits of detection (LOD) and quantification (LOQ) were found to be 9.9 × 10⁻⁹ mol L⁻¹ and 3.3 × 10⁻⁸ mol L⁻¹, respectively.

Furthermore, FLV oxidation at a glassy carbon electrode surface was used for its hydrodynamic monitoring by amperometric detection in a flow-injection system. The amperometric signal was linear with FLV concentration over the range 1.0 × 10⁻⁶ to 1.0 × 10⁻⁵ mol L⁻¹, with an LOD of 2.4 × 10⁻⁷ mol L⁻¹ and an LOQ of 8.0 × 10⁻⁷ mol L⁻¹. A sample rate of 50 injections per hour was achieved.

Both methods were validated and showed to be precise and accurate, being satisfactorily applied to the determination of FLV in a commercial pharmaceutical.

Keywords:

• adsorptive-stripping voltammetry
• amperometric detection
• flow injection analysis
• Fluvastatin
• hanging mercury drop electrode
• pharmaceutical analysis

Notes

^a GC, gas chromatography; LC, liquid chromatography; CE, capillary electrophoresis; UV, ultraviolet spectrophotometry; MS, mass spectrometric; FL, fluorimetric.

^a Concentration in µmol/L are given in parentheses.