Fluorescence Probe Enhanced Spectrofluorimetric Method for the Determination of Prulifloxacin in Pharmaceutical Formulations and Biological Fluids

Abstract

Spectrofluorimetry for the determination of prulifloxacin (PUFX) was developed based on the strong fluorescence of PUFX after adding fluorescence probe yttrium in buffer solution (pH = 6.80), and various factors of influencing fluorescence have been researched. Under the optimum conditions, the liner range was 2.0 × 10⁻⁸ to 9.1 × 10⁻⁶ mol L⁻¹ and the detection limit was 8.5 × 10⁻⁹ mol L⁻¹. The relative standard deviation was 2.1% for 11 measurements of 5.0 × 10⁻⁷ mol L⁻¹ PUFX standard solution. The mechanism of the sensitizing effect of probe was discussed. The method was applied for the determination of PUFX in actual sample; the result obtained was satisfactory.

Keywords:

• Probe
• prulifloxacin
• spectrofluorimetry
• yttrium

The authors acknowledge the support from National Natural Science Foundation of China, the Science Foundation of Huazhong University of Science and Technology (No. 0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.

Notes

^a Guo et al. 2006.

^b Changzhou Yabang Pharmaceutical Co. Ltd., Jiangsu, China (No. H20061223) and Zhicheng Pharmaceutical Co. Ltd., Heilongjiang, China (No. H20060317).

^c Zhengda Fine Pharmaceutical Co. Ltd., Jiangsu, China (No. H20060830) and Chengdu Beiter Pharmaceutical Co. Ltd., Sichuan, China (No. H20061181).

^a From a healthy male volunteer in a local hospital, Suhan, China.

^b RSD, relative standard deviation.