New MSPQC Method for Rapid Identification and Quantification of Pseudomonas aeruginosa

Abstract

A new method for the rapid identification and quantification of Pseudomonas aeruginosa using multichannel series piezoelectric quartz crystal (MSPQC) was proposed. The identification of P. aeruginosa was based on the development of acetamide broth, which can selective culture P. aeruginosa and performed perfectly in MSPQC. The quantitative detection of P. aeruginosa was based on the fact that the frequency detection time (FDT) detected by MSPQC in developed medium had a linear relationship with the logarithm of its initial concentration in the range of 10–10⁸ colony − forming units (cfu) ml^–1 (R=−0.984). The detection limit was 10 cfu ml^–1.

Keywords:

• Acetamide broth
• multichannel series piezoelectric quartz crystal (MSPQC)
• Pseudomonas aeruginosa

We are grateful to the National Natural Science Foundation of China (751214049) for financial support of this work.