Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

Qing-Ri Jin National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea

In-Wha Kim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea

Min-Koo Choi National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea

Won-Sik Shim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea

Guang-Yan Tian Department of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University, Seoul, Korea

Soohyun Lee National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea

Jonghwa Lee Korea Institute of Toxicology, Daejeon, Korea

Dae-Duk Kim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea

Suk-Jae Chung National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea

Chang-Koo Shim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, KoreaCorrespondence[email protected]

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Abstract

A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the determination of belotecan in the plasma, urine, and bile samples of rats. Belotecan was analyzed with HPLC using a C18 column with fluorescence detector. A mixture of acetonitrile–0.1 M potassium phosphate buffer at pH 2.4 (25:75, v/v) and 0.2% trifluoroacetic acid was used as the mobile phase. The lower limits of quantitation (LOQ) were 5 ng mL⁻¹ for the plasma and 5 µg mL⁻¹ for the urine and bile samples. The method has been readily applied for the routine pharmacokinetic study of belotecan in small laboratory animals.

Keywords:

This study was supported by a grant from the Korean Ministry of Science and Technology through the National Research Laboratory Program (ROA-2006-000-10290-0).

Notes

^aSD, standard deviation.

^aAUC: area under the plasma concentration time curve; CL: clearance; V_ss: apparent volume of distribution at a steady state; CL_b: biliary clearance; CL_r: renal clearance.

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Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

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Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

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