Quantification of Carcinoembryonic Antigen by Capillary Electrophoresis–Chemiluminescence Detection using Internal Standard Method

Abstract

Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis–chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal conditions, the free horseradish peroxidase (HRP)–labeled antibody (Ab∗), the bound Ab∗-antigen complex (Ab∗-Ag), and HRP used as internal standard were well separated within 5 min. The proposed method was successfully applied for the quantification of CEA in human sera obtained from both healthy persons and patients.

Keywords:

• Capillary electrophoresis
• carcinoembryonic antigen
• chemiluminescence
• internal standard method

This work was supported by the National Natural Science Foundation of China (20575056) and Henan Innovation Project for University Research Talents (2005126).

Notes

^a Migration time.

^b Peak area.

^c Average value.