Preparation and Evaluation of Propranolol-Imprinted Monolithic Stationary Phase by In Situ Technique and Application in Analysis of Propranolol in Biological Samples

Abstract

A propranolol molecule-imprinted monolithic stationary phase (MIMSP) was synthesized by in situ technique. The recognition mechanism of the polymers and the influences of some chromatographic conditions were examined by high-performance liquid chromatography (HPLC). The imprinted polymers showed much higher selectivity for β-blockers than the nonimprinted polymers (NIPs) did, which proves the successful preparation of propranolol-imprinted polymers by using an in situ technique. Then, this technique was used to prepare a molecularly imprinted polymer solid-phase extraction column to concentrate propranolol from biological samples. The results showed that the imprinted solid-phase extraction column could selectively enrich and purify propranolol from biological samples, such as plasma.

Keywords:

• Biological samples
• molecularly imprinted polymer
• monolithic stationary phase
• propranolol

Acknowledgments

This work was supported in part by Grant No. 30672551 to Qiang Fu from National Natural Science Foundation of China.

Notes

Note. “—” indicates that the polymer was too rigid to allow the mobile phase to flow through.

Note. HPLC conditions, mobile phase: acetonitrile–buffer phosphate (20 mmol·L⁻¹, pH 60:40, V/V); flow rate: 1.0 ml·min⁻¹; detection wavelength: 230 nm.

Notes. HPLC conditions, mobile phase: acetonitrile–buffer phosphate (20 mmol·L⁻¹, pH 4.0, 60:40, V/V); flow rate: 1.0 ml·min⁻¹; detection wavelength: 230 nm.