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Abstract
A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE)–ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g−1.
Acknowledgments
This research was supported by the National “863” High-Tech Project (2006AA10Z438), National Key Project of Scientific and Technical Supporting Programs of China (2006BAK02A09), Shanghai Commission of Science and Technology (0652nm017, 07dz19508), and Shanghai Leading Academic Discipline Project (B205). We are grateful to Prof. Tom Hsiang (University of Guelph, Canada) for the technical language editing.
Notes
a n.d. = not detected.