![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
Based on the selected monoclonal antibody, the effect of three reaction formats on sensitivity, specificity, and determination ability for real samples were investigated. The results showed the antibody-coated format was the optimal assay for parathion determination. The sensitivity of the assay was 3.20 ng/ml, with a detection limit of 0.40 ng/ml, and the assay time was 1.5 h. The average recoveries of parathion in river water, rice, cucumber, green soy bean, and cabbage were 98.56%, 89.46%, 99.25%, 118.57%, and 101.39%, respectively. In addition, when rice and cabbage extracts were analyzed by the assay and HPLC, the correlation was greater than 0.9.
This work was supported by the National Natural Science Foundation of China (30370944) and the Specialized Research Fund for the Doctoral Program of Higher Education.
Notes
N: no competitive reaction happened.
a IC50 value could not be determined accurately due to the limited solubility at high concentrations; however, it was clear that inhibition was less than 50% at 10000 ng/ml.