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Abstract
A newly synthesized copolymer, Poly(glutaraldehyde-co-pyrrole) was used to immobilize horseradish peroxidase for the construction of an amperometric biosensor for detection of phenols in a flow injection system. Infrared spectra of Poly(glutaraldehyde-co-pyrrole) showed that the incorporation of aldehyde groups into the conductive polymeric backbone was achieved. The values were found to be in the range of 0.077–0.468 mM for p-benzoquinone and 0.112–0.709 mM for catechol, at various flow rates ranging between 0.25–6 mL/min. The sensitivities at various flow rates ranged between 100–200 nA/mM for catechol and 30–100 nA/mM for p-benzoquinone. The biosensor retained its initial activity for 1 month.
The authors are grateful to “The Scientific & Technological Research Council of Turkey” (TUBITAK) for financial support of this project under grant Number 105Y130.