Molecular Imprinting-Chemiluminescence Sensor for the Determination of Amoxicillin

Abstract

The amoxicillin-imprinted polymer was synthesized with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The binding characteristic of the imprinted polymer to amoxicillin was evaluated by equilibrium binding experiments. Using the imprinted polymer as recognition material, 3-(3′-nitrophenyl)-5(2′-sulfonylphenylazo)-rhodanine (4NRASP) was synthesized by the authors and was used as chemiluminescence (CL) reagent. A novel chemiluminescence (CL) sensor for the determination of amoxicillin was developed based on the CL reaction of amoxicillin with potassium permanganate in an acidic medium. The sensor displayed excellent selectivity and high sensitivity. The linear response range of the sensor was from 5.0 × 10⁻⁹ to 1.0 × 10⁻⁶ g · mL⁻¹ (r = 0.9985) and the detection limit was 1.3 × 10⁻⁹ g · mL⁻¹. The relative standard deviation for the determination of 1.0 × 10⁻⁷ g · mL⁻¹ amoxicillin solution was 1.7% (n = 11). The sensor was applied to the determination of amoxicillin in urine samples with satisfactory results.

Keywords:

• Amoxicillin
• Chemiluminescence (CL)
• Flow injection
• Molecularly imprinted polymer (MIP)
• Rhodanine

This work was financially supported by the Natural Science Research Foundation of Shandong Province, China (No. Y2007B07); the Postgraduate Innovation Program of Shandong Province (SDYY08030); the National Natural Science Foundation of People's Republic of China (No. 30700535), and the National Eleventh Five-Year Plan, China (No. 2006BAJ03A09).

Notes

∗Average of six measurements.