Pico-Level Monitoring of Ampicillin by Using a Novel Cerium Fluorescence Probe

Abstract

In this work, for the first time, a simple and sensitive fluorimetric method for low level determination of ampicillin, a beta-lactam antibiotic drug, using a cerium quenching fluorescence signal was developed. Among the lanthanide ions, cerium has a broad band spectrum in 300–400 nm which is due to the 5d to 4f allowed transition. The employed methodology is based on the formation of a complex formed between Ce³⁺ and ampicillin molecule. The detection limit was 0.0115 ng mL⁻¹ (33 × 10⁻¹² mol L⁻¹).

Keywords:

• Ampicillin
• Cerium
• Fluorescence quenching
• Probe

Notes

*The results are based on triplicates measurements.