Abstract
A novel chemiluminescence (CL) method is developed for determination of ofloxacin and levofloxacin with Ag(III) complex in H2SO4 solution medium. The CL intensity is proportional to drug concentration in a wider range with a correlation coefficient of 0.999. The limit of detection (s/n = 3) for ofloxacin and levofloxacin was 5.3 × 10−9 g ml−1 and 8.3 × 10−9 g ml−1, respectively, and their recoveries from urine and serum samples were in the range of 90.1–112% with the RSDs of 1.0–2.8%. The proposed method was applied for analysis of real samples with satisfactory result. The possible CL mechanism was discussed.
This work was supported by the Science Foundation Education Office of Hebei Province (B2008000583).
Notes
*n = 5.
*n = 5, **no detected.