Determination of Albumin Using the Chemiluminescence of Tb³⁺/K₂S₂O₈ System

Abstract

A new chemiluminescence system of Tb³⁺/K₂S₂O₈ was developed for the determination of albumin. Some experimental conditions were examined and optimized. The linear ranges of the calibration curves are 5.0 × 10⁻⁹–5.0 × 10⁻⁶ mol/L for bovine serum albumin, 5.0 × 10⁻⁸–1.0 × 10⁻⁵ mol/L for human serum albumin and 2.5 × 10⁻⁷–1.0 × 10⁻⁵ mol/L for ovalbumin, and the corresponding detection limits are 1.9 × 10⁻⁹ mol/L, 1.5 × 10⁻⁸ mol/L, and 1.5 × 10⁻⁷ mol/L, respectively. The method was applied to the determination of albumin in human serum samples, and the results were in agreement with those obtained by the bromcresol green method. The relative errors for the analytical results were from −2.0% to 4.3%.

Keywords:

• Albumin
• Chemiluminescence
• Lanthanide ions

Notes

The RSDs were estimated under 1.0 × 10⁻⁷ mol/L for BSA, 5.0 × 10⁻⁷ mol/L for HSA and 5.0 × 10⁻⁶ mol/L for OVA (n = 11).