Resonance Light Scattering Method for Microdetermination of Proteins Based on Aggregation of Au Nanoparticles

Abstract

By using a resonance light scattering (RLS) technique, a highly sensitive method for protein determination based on the aggregation of Au nanoparticles on protein template is described. For the Au nanoparticles of 15 nm, the detection limit of bovine serum albumin was 5.0 ng/mL and the linear range was 10–300 ng/mL. The experimental results indicated that various metal ions do not interfere with this assay. The proposed RLS assay exhibited lower variation in response signals for the same weight of different proteins and showed satisfactory results when it was used for determination of proteins in human serum.

Keywords:

• Au nanoparticles
• Proteins
• Resonance light scattering

Acknowledgments

We are grateful to Dr. Zhengping Li from Hebei University of China for critical reading of the manuscript.

Notes

*I _RLS is the RLS intensity.

*Average of at least two measurements.

*The results were obtained by using 0.2 µg/mL proteins.