In Vitro Electrochemical Study on Combined Cytotoxicity of 5-Fluorouracil and Three Types of Nanoparticles Against MG-63 Cells

Abstract

The individual and combined cytotoxicity of 5-fluorouracil and the three nanoparticles, silica, selenium, and gold nanoparticles against human osteosarcoma MG-63 cells were investigated by using cyclic voltammetry and microscopic observation, respectively. It is found that both the drug and the nanoparticles were able to inhibit cell proliferation in a dose-dependent way and the inhibition efficiency of Au NPs was most prominent. The combination of 5-FU with high-concentration SiO₂ NPs and Se NPs presented a simple-addition and synergistic effect, respectively. The present work may be conducive for the exploration of the applications of nanoparticles in biomedicine and cancer treatment.

Keywords:

• 5-Fluorouracil
• Cyclic voltammetry
• Cytotoxicity
• MG-63 cells
• Three nanoparticles

Acknowledgments

This work was supported by the National Science Foundation of China (20905025, 90713018, 20975037, and 20705008), the State Special Scientific Project on Water Treatment (2009ZX07212-001-06), the Scientific Research Fund of Hunan Provincial Education Department (09B062), and the Doctor Startup Fund of Hunan Normal University (090607).