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Analytical Letters Volume 6, 1973 - Issue 12
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SPECTROSCOPY

The Spectrophotometric Determination of Vitamin a Using Iodine

F. Al-sulimany Department of Chemistry, University of Birmingham, PO Box 363, Birmingham, B15 2TT, U.K.
&
Alan Townshend Department of Chemistry, University of Birmingham, PO Box 363, Birmingham, B15 2TT, U.K.
Pages 1029-1037 | Received 03 Oct 1973, Accepted 26 Oct 1973, Published online: 05 Dec 2006
 
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Abstract

Methods are described for the spectrophotometric determination of vitamin A (0.15–12.5 μg) using iodine as the chromogenic reagent. The reaction is most sensitive in chloroform. Vitamin D2 and ß-carotene do not interfere if the analysis is carried out in 1, 2 -dichloroethane.

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