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Analytical Letters Volume 6, 1973 - Issue 3
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ELECTROCHEMISTRY

Fluorometric Method for Determination of Transketolase Activity

R. Cochran Department of Chemistry, Louisiana State University in New Orleans, New Orleans, Louisiana, 70122
,
S. S. Kuan Department of Chemistry, Louisiana State University in New Orleans, New Orleans, Louisiana, 70122
&
G. G. Guilbault Department of Chemistry, Louisiana State University in New Orleans, New Orleans, Louisiana, 70122
Pages 235-243 | Received 29 Jan 1973, Accepted 09 Feb 1973, Published online: 06 Dec 2006
 
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Abstract

A new method for rapid and accurate determination of transketolase is studied. The reaction is monitored by coupling the system with the glyceraldehyde-3-phosphate dehydrogenase system and flurometrically measureing the production of NADH. The assay is rapid and convenient. It responds linearly to transketolase activity as low as 0.1 units in three ml of reaction mixture.

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