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Original Articles

Detection of Nanomole Quantities of Reducing Sugar by Liquid Chromatography

, , , &
Pages 363-368 | Received 21 Feb 1973, Accepted 02 Mar 1973, Published online: 06 Dec 2006
 

Abstract

The alkaline ferricyanide and the Park-Johnson's methods were studied in order to determine glucose oligomers separated by liquid chromatography in nanomole quantities. In the alkaline ferricyanide method, high sensitivity was obtained by measuring ultraviolet absorbance of the reduced reagents. The presence of some kinds of salts or alcohols did not affect the colour development of the reagents. The applicability of the present measuring system to an automatic sugar analyzer has been demonstrated by examining several glucose oligomers.

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