Simultaneous Quantitation of Prostaglandins F_1α. (PGF_1α) and PGF_2aL by Selected Ion Recording with a Deuterated Internal Standard

Abstract

A gas chromatographic-mass spectrometric method for the simultaneous measurement of PGF_1α, and PGF_2α at the ng level is reported. The internal standard was [3, 3, 4, 4-D] PGF_2α. The method involves conversion of the substrates to their methyl ester-trimethyl-silyl ether (ME-TMS) derivatives and selected ion recording. The standard curves were reproducible and linear between 0. 5 and 40 ng (1. 4 to 112 picomole). The regression characteristics of the standard curves for each PGF were virtually identical to those of the analogous curves from separate determinations.

Key Words:

• PGF_1α
• PGF_2α
• simultaneous measurement
• GC-MS