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Abstract
A spectrofluorimetric method for protein estimation based on the use of l-4-diamino-2-3-dich1oro-anthraquinone as a fluorogenic reagent is presented and applied to renin study. The method is optimized and the variables that influence the development of the fluorescence are studied. Data obtained by this method for Renal Renin Activity (RRA) are compared with those obtained by Bioassay (BA) and Radioinmunoassay (RIA), finding that this spectrofluorimetric method is a good one related to Radioinmunoassay.