Electrochemical Cleanup of Solutions for UV-Visible Assays Involving Low Absorbance or Dilute Samples

Abstract

In low-absorbance assays, the necessity for eliminating ultraviolet and visible absorbing impurities in buffering agents and detergents is discussed. The method proposed here involves electrochemical reduction of the interfering material. An example of the elimination of an artifact in an enzyme assay is presented.

Key Words:

• Low absorbance
• Ultraviolet
• Visible
• Electrochemical reduction
• buffers
• detergents
• dioldehydrase