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Analytical Letters Volume 20, 1987 - Issue 8
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PHARMACEUTICAL ANALYSIS

31P NMR and HPLC Investigations of the Cyclophosphamide Metabolite, Phosphoramide Mustard

R. W. Hardy Research Institute, Hospital for Sick Children, Toronto, M5G 1X8; Departments of Biochemistry and Clinical Biochemistry, University of Toronto, Toronto, M5S 1A8, Ontario, Canada
,
P. E. Fraser Research Institute, Hospital for Sick Children, Toronto, M5G 1X8; Departments of Biochemistry and Clinical Biochemistry, University of Toronto, Toronto, M5S 1A8, Ontario, Canada
,
C. M. Deber Research Institute, Hospital for Sick Children, Toronto, M5G 1X8; Departments of Biochemistry and Clinical Biochemistry, University of Toronto, Toronto, M5S 1A8, Ontario, Canada
&
SJ. Soldin Research Institute, Hospital for Sick Children, Toronto, M5G 1X8; Departments of Biochemistry and Clinical Biochemistry, University of Toronto, Toronto, M5S 1A8, Ontario, Canada
Pages 1303-1314 | Received 19 May 1987, Accepted 28 May 1987, Published online: 05 Dec 2006
 
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Abstract

Phosphoramide mustard (PM), a key active metabolite of the widely used anticancer drug cyclophosphamide (CP), can exist in several closely-related ionized, cyclized and substituted forms. We have developed a high pressure liquid chromatographic (HPLC) method for analysing serum concentrations of PM in order to relate these serum concentrations to toxicity and efficacy of treatment of CP. 31p NMR spectroscopy is used to verify the HPLC peak homology of the proposed PM peak.

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Related Research Data

Synthese und Abbau cytostatisch wirksamer cyclischer N‐Phosphamidester des Bis‐(β‐chloräthyl)‐amins
Source: Wiley
Phosphorus-31 NMR kinetic studies of the intra- and intermolecular alkylation chemistry of phosphoramide mustard and cognate N-phosphorylated derivatives of N,N-bis(2-chloroethyl)amine
Source: American Chemical Society (ACS)
Phosphorus-31 NMR investigations of phosphoramide mustard: evaluation of pH control over the rate of intramolecular cyclization to an aziridinium ion and the hydrolysis of this reactive alkylator
Source: American Chemical Society (ACS)

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