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Analytical Letters Volume 22, 1989 - Issue 4
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Pharmaceutical Analysis

Determination of Bile Acids In Pharmaceutical Dosage Forms By Hplc

S. Scalia Department of Pharmaceutical Sciences, University of Ferrara, Via Scandiana 21, 44100, Ferrara, Italy
,
P. Pazzi Department of Medicine, Arcispedale S. Anna, 44100, Ferrara, Italy
&
M. Guaneri Department of Pharmaceutical Sciences, University of Ferrara, Via Scandiana 21, 44100, Ferrara, Italy
Pages 915-927 | Received 05 Dec 1988, Accepted 10 Jan 1989, Published online: 23 Oct 2006
 
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Abstract

A rapid reversed-phase high-performance liquid chromatographic assay was developed for chenodeoxycholic acid and ursodeoxycholic acid in commercially available capsule and tablet formulations. A Supelcosil LC-18-DB column with UV detection at 210 nm and a methanol-acetate buffer eluent were used. Individual dosage forms were disintegrated in the mobile phase and an aliquot of the resulting suspension was filtered for the HPLC analysis. Recovery of the drugs from spiked placebo preparations was quantitative. the method was tested for linearity and specificity and was found to be simple, accurate and reproducible.

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