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Abstract
Rapid, precise, accurate and specific high-performance liquid chromatographic and first-derivative spectrophotometric procedures were described for the simultaneous analysis of amitriptyline and chlordiaze-poxide in two-component capsule formulations.
A reversed phase Micropack MCH-5 (CI8) column with a mobile phase composed of acetonitrile/water in a ratio (50:50) at pH3 and containing 0.01 M sodium-n-octane sulfonate separated amitriptyline, chlordiazepoxide and diazepam (internal standard). the drugs were detected at 230 nm at ambient temperature. the trough amplitudes in the first derivative spectrophotometric spectra at 245 nm and 282 nm were selected to determine amitriptyline and chlordiazepoxide.
Commercial capsules and laboratory-prepared mixtures containing both drugs in different proportions were assayed using the developed methods. the results were of comparable accuracy as indicated by the statistical analysis of data using t-test and F-test.