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Abstract
In the present work the influence of carbonic anhydrase (CA) on the gaseous exchanges of CO2 and NH3 in a buffered solution has been studied by means of a potentiometric technique involving the use of an urease sensor, whose sensing element was a commercial pNH3 gas sensing electrode. As pointed out in previous works, suitable experimental conditions were chosen in order to have a speed of CO2 diffusion sharply enhanced by CA. In particular the analytical aspect of NH3 production from a buffered urea solution, in the presence and in the absence of CA, is considered.
The results obtained in the present work indicate a further possible application of this system to the study of living organism and tangibly support all the previous studies presented on this topic.