Abstract
A sensitive flow injection chemiluminescence (CL) method is proposed for the determination of bovine serum albumin (BSA) using Copper(II)-Alizarin Red S (ARS) complex as an efficient chemiluminescent probe. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II)-ARS in the luminol-H2O2 CL system. Under the selected conditions, the CL intensity is linear with the concentration of BSA in the range of 5.0 × 10−11 to 1.0 × 10−9 mol · L−1. The detection limit was 2.0 × 10−11 mol · L−1. The method is successfully applied to the determination of protein in urine.
Acknowledgments
This paper was submitted as part of a Special Issue on Flow Injection Analysis.
The authors acknowledge the support from the National Natural Science Foundation of China (No. 20505004), Outstanding Adult-young Scientific Research Encouraging Foundation of Shandong Province (No. 2008BS03015), and the Doctoral Found of QUST (No. 0022235).