Abstract
Anatoxin-a is a neurotoxic alkaloid which is produced by various cyanobacteria genera and it is highly toxic to animals. Anatoxin-a risk evaluation is not fully determined yet. Therefore, it is imperative to implement detection and quantification methods in the various possible matrices. The recent worldwide shortage of acetonitrile, as well as environmental reasons, demand an urgent alternative to this high-performance liquid chromatography (HPLC) mobile phase solvent. This work describes an internal study that validated the measuring method of anatoxin-a in water and lyophilized trout matter by means of HPLC with fluorimetric detection (FLD) utilizing methanol as mobile phase.
Acknowledgments
The authors wish to thank to the Portuguese Foundation for Science and Technology (FCT) for funding support (grant SFRH/BPD/37804/2007).
Notes
a µ = χ ± t(n−1) (SD/√n); Ttab(n = 4) = 2.26; Ttab(n = 3) = 4,30; 95%.
a µ = χ ± t(n−1) (SD/√n); Ttab(n = 4) = 2.26; Ttab(n = 3) = 4,30; 95%.
a Three series of the toxicity study.
b µ = χ ± t(n−1) (SD/√n); Ttab(n = 3) = 4,30; 95%.