Technological Advancements in Mass Spectrometry and Its Impact on Proteomics

Abstract

Amalgamation of mass spectrometry (MS) and proteomics has led to the most awaited technological inventions such as discovery of clinically potential biomarkers and generation of effective drugs. This review focuses on the synergistic growth in MS instrumentation, proteomics and its impact on biomedical sciences. Novel ionization methods: surface enhanced laser desorption ionization, electrospray assisted laser desorption ionization, desorption electrospray ionization, laser diode thermal desorption are discussed. Different mass analyzers: ion trap, time-of-flight, Fourier transform ion cyclotron resonance and their applications are outlined. New ion fragmentation techniques: electron capture dissociation, electron transfer dissociation, infrared multiphoton dissociation and their attributes are described.

Keywords:

• Ion fragmentation
• Ionization
• Mass analysis
• Mass spectrometry
• Proteomics

Notes

^a Triple quadrupole;

^b Double quadrupole-TOF.

^a Quadrupole ion trap.

^b Triple quadrupole.

^c Double quadropole-linear ion trap.

^d Tandem TOF.

^e Double quadrupole-TOF.

^f Linear trap quadrupole-Orbitrap.