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Abstract
We fabricated a two-dimensional (2D) molecularly imprinted sol-gel thin film-coated quartz crystal microbalance (QCM) for the rapid detection of staphylococcal enterotoxin B (SEB) by combining organosilanes and the template protein SEB on the surface of piezoelectric quartz crystal (PQC) Au-electrode by in-situ immobilization. The detection process was monitored by the QCM's frequency shift (Δf). The working range of this method was 1.0 × 10−1–1.0 × 103 µg/mL. The detection limit was 6.1 ng/mL, which was lower than that of the PQC immunosensor, and the detection period was within 0.5 h. The reproducibility of the imprinted film-coated QCM was satisfactory due to no significant statistical difference (P > 0.05) in the rapid detection of SEB between intra- and inter-batch. The selectivity of the imprinted sol-gel film showed that it could discriminate the template molecule from its analogues and other guest molecules. Compared with immunochip, the imprinted film-coated QCM is more advantageous in terms of simplicity, rapidity, low cost, and sensitivity. Moreover, in real sample analysis, the recoveries of this method were 89.4–106.63%, which can be considered a favorable and applicable method for the rapid determination of SEB in real samples.
Acknowledgments
The authors gratefully acknowledge the financial support provided by the National Nature Science Foundation of China (No. 30800915), the National Science and Technology Supporting Program of China (No. 2009BADB9B03-Z05), the National High Technology R&D Program of China (No. 2010AA06Z302) and the Science and Technology Program of Tianjin (No. 09ZCKFSH02700).
Notes
Compared * to Δ, P > 0.05.
a Undetectable concentrations or no results.
This paper was submitted as part of a Special Issue on Biosensors organized by Dr. Yu Lei of the University of Connecticut.