Effect of Selected Microorganisms on Fusarium Toxins Production

Abstract

The aim of this study was to examine the effect of selected microorganisms on mycotoxins production by molds of the genus Fusarium, namely HT-2 and T-2 toxins. Appropriate nutritive media were inoculated with test microorganisms (Rhodotorula spp., Leuconostoc spp., Pantoea agglomerans), subsequently inoculated with Fusarium molds, then incubated under various conditions. Content of Fusarium mycotoxins in individual samples was determined using HPLC/MS/MS. Separation of mycotoxins was performed on a C₁₈ stationary phase column using gradient elution. Total analysis time was less than 20 minutes. In examining the effect of accompanying microflora on the production of HT-2 and T-2 toxins, a decrease in production of both mycotoxins was observed under various experimental conditions. Greatest inhibitory effect was observed in the presence of Pantoea agglomerans CCM 298 bacteria. It was found that the amount of HT-2 and T-2 toxins produced by the examined mold strains also depends on cultivation conditions and the nutritive medium used.

Keywords:

• Fusarium
• HPLC/MS/MS
• HT-2 toxin
• T-2 toxin

Acknowledgments

This work was conducted with financial support from the Ministry of Education, Youth and Sports of the Czech Republic (Project MSMT 0021627502) and from the Grant Agency of the Czech Republic (Project No. GACR 203/09/0148).

Notes

*Solvent A: 0.1% formic acid in water.

**Solvent B: 0.1% formic acid in acetonitrile.

*Overlaying 1 mL of inoculate with cell density of 6 × 10⁸ CFU mL⁻¹.

**Spreading 0.1 mL of inoculate with cell density of 6 × 10⁸ CFU mL⁻¹.

*Overlaying 1 mL of inoculate with cell density of 6 × 10⁸ CFU mL⁻¹.

**Spreading 0.1 mL of inoculate with cell density of 6 × 10⁸ CFU mL⁻¹.