Simultaneous Determination of Organotin Compounds in White Wine by Gas Chromatography-Mass Spectrometry

Abstract

A simple, reliable, and effective analytical method was developed for the simultaneous determination of five organotin compounds (OTCs) including monobutyltin trichloride dibutyltin dichloride tributyltin chloride tetrabutyltin and triphenyltin chloride in white wines. The OTCs were derivatized with sodium tetraethylborate (NaBEt₄), and their derivatives were extracted by liquid-liquid extraction (LLE) into n-hexane. The experimental variables, such as type and volume of extraction solvents, amount of derivatization reagent NaBEt₄ and extraction time were optimized. The determination of ethylated derivatives of OTCs in the final extracts was carried out by gas chromatography-mass spectrometry (GC-MS). Under optimized conditions, good linearity was observed when analytical concentrations were in the range of 0.01–4.0 µg · mL⁻¹, the linearity correlation coefficients were between 0.9982 and 0.9987, with the LODs in the range of 0.2–3.0 µg · L⁻¹, and the LOQs varied from 0.6 to 10.0 µg · L⁻¹. The obtained recoveries were in the range of 78.0–120.0%, with the relative standard deviations equal to or lower than 8.1%. This method was applied to the determination of OTCs in white wines with satisfactory results.

Keywords:

• GC-MS
• Liquid-liquid extraction
• Organotin compounds
• White wine

Acknowledgments

The financial support of this study from the Natural Science Foundation of China (20965005), the Research Program of State Key Laboratory of Food Science and Technology in Nanchang University (SKLF-TS-200918), the Objectives-oriented Project for the State Key Laboratory of Food Science and Technology in Nanchang University (SKLF-MB-201002), and the Science and Technology Planning Project of Jiangxi Province (2008BB22400) are gratefully acknowledged.

Notes

^a Data are shown as mean ± SD.

^a ND, not detected (<LOD).

^b Data were shown as mean ± SD.